Fluorescently Labeled Peptides

Fluorescently labeled peptides are used for in vivo biomedical imaging, localization studies and protein binding. In peptides SH group of cysteine and NH2 group lysine or N-terminus are reactive sites for fluorescent label. Biodyes generally coupled with cysteine through maleimide functionality in liquid phase alternately click chemistry can works well. The dyes can coupled either through NHS-ester or directly during the SPPS. Following commonly used Biodyes used for conjugation with peptides.